Development of a High-Throughput Opsonophagocytic Assay for the Determination of Functional Antibody Activity Against Streptococcus Pyogenes Using Bioluminescence

J Microbiol Methods. 2017 Mar;134:58-61. doi: 10.1016/j.mimet.2017.01.010. Epub 2017 Jan 21.

Abstract

The lack of standardised protocols for the assessment of functional antibodies has hindered Streptococcus pyogenes research and the development of vaccines. A robust, high throughput opsonophagocytic bactericidal assay to determine protective antibodies in human and rabbit serum has been developed that utilises bioluminescence as a rapid read out.

Keywords: Bactericidal assay; Bioluminescence; Correlates of protection; Group A Streptococcus (GAS); Lancefield assay; Protective antibodies.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Bacterial / blood*
  • Antibodies, Bacterial / immunology*
  • Enzyme-Linked Immunosorbent Assay
  • High-Throughput Screening Assays*
  • Humans
  • Immunologic Tests / methods*
  • Luminescent Measurements
  • Phagocytosis*
  • Rabbits
  • Streptococcal Infections / diagnosis
  • Streptococcal Infections / microbiology
  • Streptococcus pyogenes / immunology*

Substances

  • Antibodies, Bacterial