Evidence for unusually short tubulin mRNA leaders and characterization of tubulin genes in Giardia lamblia

Mol Biochem Parasitol. 1989 Aug;36(1):87-99. doi: 10.1016/0166-6851(89)90204-1.

Abstract

We have cloned alpha- and beta-tubulin cDNAs from Giardia lamblia and have used these to determine the gene copy number in the organism and to isolate alpha- and beta-tubulin genomic clones. Studies of the gene organization demonstrate that two copies of beta-tubulin are linked in a head-to-head configuration. The DNA from these two copies and that from one alpha-tubulin copy has been sequenced upstream of the translation initiation codon, and analyzed for consensus to typical eukaryotic promoter sequences. Characterization of the alpha- and beta-tubulin mRNAs in this parasite by primer extension and S1 nuclease mapping has revealed an unusually short (6 nucleotides) 5' untranslated region.

MeSH terms

  • Animals
  • Base Sequence
  • Blotting, Northern
  • Cloning, Molecular
  • DNA / analysis
  • DNA / isolation & purification
  • DNA Probes
  • Gene Amplification
  • Giardia / genetics*
  • Molecular Sequence Data
  • Multigene Family
  • Protein Sorting Signals / genetics*
  • RNA, Messenger / analysis*
  • Restriction Mapping
  • Tubulin / genetics*

Substances

  • DNA Probes
  • Protein Sorting Signals
  • RNA, Messenger
  • Tubulin
  • DNA

Associated data

  • GENBANK/J04646
  • GENBANK/J04647
  • GENBANK/J04648