MiR-125b inhibits anaplastic thyroid cancer cell migration and invasion by targeting PIK3CD

Biomed Pharmacother. 2017 Apr;88:443-448. doi: 10.1016/j.biopha.2016.11.090. Epub 2017 Jan 22.

Abstract

Emerging evidences indicate that dysregulated microRNAs are implicated in the process of tumorigenesis and progression. The miRNA-125b (miR-125b) is downregulated and identified as tumor supressor in various cancers including thyroid cancer. However, the role and mechanism of miR-125b in anaplastic thyroid cancer (ATC) migration and invasion remain unknown. In the present study, the expression levels of miR-125b were downregulated and the expression levels of phosphoinositide 3-kinase catalytic subunit delta (PIK3CD) were upregulated in ATC tissues and cell lines. Moreover, miR-125b expression was negatively related to PIK3CD expression in ATC tissues. A computational search and luciferase assay identified PIK3CD as a direct target of miR-125b in ATC and PIK3CD expression was downregulated by miR-125b in ATC cells. In terms of function, miR-125b repressed migration and invasion of ATC cells, whereas PIK3CD overexpression reversed this effect. Furthermore, we showed that exogenous miR-125b decreased the PI3K, phospho-Akt, and phospho-mTOR expression in ATC cells. In conclusion, these results indicated that miR-125b suppressed ATC cell migration and invasion by targeting PIK3CD expression, and suggested novel potential therapeutic targets for the treatment of ATC.

Keywords: ATC; Invasion; Migration; PIK3CD; miR-125b.

MeSH terms

  • Base Sequence
  • Cell Line, Tumor
  • Cell Movement / genetics*
  • Class I Phosphatidylinositol 3-Kinases / metabolism*
  • Gene Expression Regulation, Neoplastic
  • Humans
  • MicroRNAs / genetics
  • MicroRNAs / metabolism*
  • Neoplasm Invasiveness
  • Signal Transduction / genetics
  • Thyroid Carcinoma, Anaplastic / genetics*
  • Thyroid Carcinoma, Anaplastic / pathology*
  • Thyroid Neoplasms / genetics*
  • Thyroid Neoplasms / pathology*

Substances

  • MIRN125 microRNA, human
  • MicroRNAs
  • Class I Phosphatidylinositol 3-Kinases
  • PIK3CD protein, human