Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
, 31 (6)

Evaluation of Kodecytes Using Function-Spacer-Lipid Constructs as a Survey Material for External Proficiency Testing for ABO Subgrouping

Affiliations

Evaluation of Kodecytes Using Function-Spacer-Lipid Constructs as a Survey Material for External Proficiency Testing for ABO Subgrouping

Young Ae Lim et al. J Clin Lab Anal.

Abstract

Background: It is not easy to find natural red blood cells (RBCs) with weak A (Aw ) or weak B phenotype (Bw ) for use as quality controls in ABO subgroup testing (subgrouping). The aim of this study was to prepare RBC kodecytes with synthetic blood group A and/or B function-spacer-lipid (FSL) constructs and to evaluate the possibility of using such kodecytes as a survey material for an external proficiency test (PT) to improve the quality of subgroup analysis.

Methods: Three types of survey samples, including O phenotype RBCs and A kodecytes with Aw (0.02 mg/mL FSL-A solution) and B kodecytes with Bw (0.15 mg/mL FSL-B solution) were sent to 53 laboratories for an educational trial of PT for subgrouping. Cell typing was done using the manual tube technique.

Results: Forty-three laboratories responded, and the re-activities of the survey samples varied from 0 to 4+ against anti-A and anti-B monoclonal reagents(MoAbs). Twenty-nine laboratories (67%) correctly grouped the Bw kodecytes as Bw . Fifteen (35%), 21 (48%), and 6 (13%) laboratories grouped the Aw kodecytes as Aw , A2 , and O phenotypes, respectively. The anti-A MoAb clone affects the results of cell typing for Aw kodecytes. The stability of kodecytes was similar to that of natural O RBCs during storage.

Conclusion: Our kodecytes were useful as a survey material, and the survey results showed the necessity of materials for PT for subgrouping to improve the quality of laboratory analysis regardless of the different reactions according to the MoAb.

Keywords: blood groups; immunohaematology; proficiency testing; quality control.

Figures

Figure 1
Figure 1
Three types of survey materials used for external proficiency testing (a) for ABO subgrouping, including weak A phenotype (Aw) and weak B phenotype (Bw) with anti‐A, and anti‐B MoAbs (Millipore, Livingston, UK), using the tube technique (b)
Figure 2
Figure 2
Relationship between grade of kodecyte agglutinations against MoAbs (Millipore, Livingston, UK) and concentration of function‐spacer‐lipid (FSL)‐A (n=40) and FSL‐B (n=24) working solutions
Figure 3
Figure 3
Changes in the mean of the sum of grades of kodecyte agglutinations (P=.392, a) and in the average levels of plasma hemoglobin (P<.001, b) according to storage days in the three types of preservative solution as assessed by multivariate (Wilks’ Lambda) repeated measures ANOVA testing (n=5). As, Alsever's solution; Ec, Ec‐stabilizing solution: PBS, Phosphate‐buffered saline
Figure 4
Figure 4
Histograms of A and B antigens of kodecytes and natural RBCs against monoclonal antibodies (MoAbs) used for ABO cell typing. (a) RBC O, A2, and A3 phenotypes were compared with A kodecytes (Aw) with 0.02 mg/mL of FSL‐A solution against anti‐A MoAb. (b) Blood groups B and O were compared with B kodecytes (Bw) with 0.15 mg/mL of FSL‐B solution against anti‐B MoAb. The mean (median) fluorescent intensity ratios are as follows: A2 phenotype, 35.14 (37.97); A3 phenotype, 11.50 (2.91); A kodecytes, 4.16 (5.60); B phenotype, 127.04 (133.22); B kodecytes, 3.55 (3.37); and B3 phenotype, 3.10 (1.07)

Similar articles

See all similar articles

Cited by 1 PubMed Central articles

MeSH terms

LinkOut - more resources

Feedback