The Long Non-Coding RNA RHPN1-AS1 Promotes Uveal Melanoma Progression

Int J Mol Sci. 2017 Jan 23;18(1):226. doi: 10.3390/ijms18010226.


Increasing evidence suggests that aberrant long non-coding RNAs (lncRNAs) are significantly correlated with the pathogenesis, development and metastasis of cancers. RHPN1 antisense RNA 1 (RHPN1-AS1) is a 2030-bp transcript originating from human chromosome 8q24. However, the role of RHPN1-AS1 in uveal melanoma (UM) remains to be clarified. In this study, we aimed to elucidate the molecular function of RHPN1-AS1 in UM. The RNA levels of RHPN1-AS1 in UM cell lines were examined using the quantitative real-time polymerase chain reaction (qRT-PCR). Short interfering RNAs (siRNAs) were designed to quench RHPN1-AS1 expression, and UM cells stably expressing short hairpin (sh) RHPN1-AS1 were established. Next, the cell proliferation and migration abilities were determined using a colony formation assay and a transwell migration/invasion assay. A tumor xenograft model in nude mice was established to confirm the function of RHPN1-AS1 in vivo. RHPN1-AS1 was significantly upregulated in a number of UM cell lines compared with the normal human retinal pigment epithelium (RPE) cell line. RHPN1-AS1 knockdown significantly inhibited UM cell proliferation and migration in vitro and in vivo. Our data suggest that RHPN1-AS1 could be an oncoRNA in UM, which may serve as a candidate prognostic biomarker and target for new therapies in malignant UM.

Keywords: RHPN1-AS1; lncRNA; migration; uveal melanoma.

MeSH terms

  • Cell Line, Tumor
  • Cell Movement / genetics
  • Cell Proliferation
  • Cytoplasm / genetics
  • Disease Progression*
  • Down-Regulation / genetics
  • Gene Expression Profiling
  • Gene Expression Regulation, Neoplastic
  • Gene Knockdown Techniques
  • Gene Ontology
  • Genome, Human
  • Humans
  • Melanoma / blood supply
  • Melanoma / genetics*
  • Melanoma / pathology*
  • Neoplasm Invasiveness
  • Neovascularization, Pathologic / genetics
  • RNA, Long Noncoding / genetics*
  • RNA, Small Interfering / metabolism
  • Up-Regulation / genetics
  • Uveal Neoplasms / blood supply
  • Uveal Neoplasms / genetics*
  • Uveal Neoplasms / pathology*
  • Xenograft Model Antitumor Assays


  • RNA, Long Noncoding
  • RNA, Small Interfering
  • long noncoding RNA RHPN1-AS1, human

Supplementary concepts

  • Uveal melanoma