CRISPR/Cas 9 genome editing and its applications in organoids

Am J Physiol Gastrointest Liver Physiol. 2017 Mar 1;312(3):G257-G265. doi: 10.1152/ajpgi.00410.2016. Epub 2017 Jan 26.


Organoids are three-dimensional (3D) structures derived from adult or embryonic stem cells that maintain many structural and functional features of their respective organ. Recently, genome editing based on the bacterial defense mechanism CRISPR/Cas9 has emerged as an easily applicable and reliable laboratory tool. Combining organoids and CRISPR/Cas9 creates exciting new opportunities to study organ development and human disease in vitro. The potential applications of CRISPR in organoids are only beginning to be explored.

Keywords: CRISPR/Cas9; gene editing; organoid.

Publication types

  • Review

MeSH terms

  • Clustered Regularly Interspaced Short Palindromic Repeats*
  • Gene Editing*
  • Humans
  • Organoids*