Ror2 Signaling Regulates Golgi Structure and Transport Through IFT20 for Tumor Invasiveness

Sci Rep. 2017 Jan 26;7(1):1. doi: 10.1038/s41598-016-0028-x.

Abstract

Signaling through the Ror2 receptor tyrosine kinase promotes invadopodia formation for tumor invasion. Here, we identify intraflagellar transport 20 (IFT20) as a new target of this signaling in tumors that lack primary cilia, and find that IFT20 mediates the ability of Ror2 signaling to induce the invasiveness of these tumors. We also find that IFT20 regulates the nucleation of Golgi-derived microtubules by affecting the GM130-AKAP450 complex, which promotes Golgi ribbon formation in achieving polarized secretion for cell migration and invasion. Furthermore, IFT20 promotes the efficiency of transport through the Golgi complex. These findings shed new insights into how Ror2 signaling promotes tumor invasiveness, and also advance the understanding of how Golgi structure and transport can be regulated.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carrier Proteins / genetics
  • Carrier Proteins / metabolism*
  • Cell Line, Tumor
  • Cell Movement
  • Golgi Apparatus / metabolism*
  • Humans
  • Mesenchymal Stem Cells / ultrastructure
  • Microtubules / metabolism
  • Neoplasm Invasiveness / pathology*
  • Podosomes / pathology
  • RNA, Small Interfering / genetics
  • Receptor Tyrosine Kinase-like Orphan Receptors / metabolism*
  • Signal Transduction

Substances

  • Carrier Proteins
  • IFT20 protein, human
  • RNA, Small Interfering
  • Receptor Tyrosine Kinase-like Orphan Receptors