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. 2017 Apr 3;13(4):770-771.
doi: 10.1080/15548627.2016.1278093. Epub 2017 Jan 27.

Single-cell RNA Sequencing Highlights Transcription Activity of Autophagy-Related Genes During Hematopoietic Stem Cell Formation in Mouse Embryos

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Free PMC article

Single-cell RNA Sequencing Highlights Transcription Activity of Autophagy-Related Genes During Hematopoietic Stem Cell Formation in Mouse Embryos

Yongfei Hu et al. Autophagy. .
Free PMC article

Abstract

Accumulating evidence has demonstrated that macroautophagy/autophagy plays an essential role in self-renewal and differentiation in embryonic hematopoiesis. Here, according to the RNA sequencing data sets of 5 population cells related to hematopoietic stem cell (HSC) formation during mouse embryogenesis (endothelial cells, PTPRC/CD45- and PTPRC/CD45+ pre-HSCs in the E11 aorta-gonad-mesonephros (AGM) region, mature HSCs in E12 and E14 fetal liver), we explored the dynamic expression of mouse autophagy-related genes in this course at the single-cell level. Our results revealed that the transcription activity of autophagy-related genes had a substantial increase when endothelial cells (ECs) specified into pre-HSCs, and the upregulation of autophagy-essential genes correlated with reduced NOTCH signaling in pre-HSCs, suggesting the autophagy activity may be greatly enhanced during pre-HSC specification from endothelial precursors. In summary, our results presented strong evidence that autophagy plays a critical role in HSC emergence during mouse midgestation.

Keywords: autophagy; hematopoiesis; hematopoietic stem cell; mouse embryos; single cell.

Figures

Figure 1.
Figure 1.
Autophagy-related gene expression dynamics during the process of HSC formation in mouse embryo at the single-cell level. (A) Heat map of differentially expressed autophagy-related genes between each of the 2 consecutive stages during the formation of HSCs in mouse embryo. (B) Hierarchical cluster of gene expression of autophagy- essential genes and NOTCH target genes in pre-HSCs (including both T1 and T2 pre-HSCs) at the single-cell level.

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  • Autophagy. doi: 10.1038/nature17997

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