Chromatographic Methods to Evaluate Nutritional Quality in Oat

Methods Mol Biol. 2017;1536:115-125. doi: 10.1007/978-1-4939-6682-0_8.


Oats (A. sativa L.) have an important and positive role in human diet and health. The health benefits of oats are attributed to its multifunctional characteristic and nutritional profile, being an important source of soluble dietary fiber, well-balanced proteins, unsaturated fatty acids, vitamins, essential minerals, and a good source of natural antioxidants. These antioxidants include the avenanthramides (Avns) and avenalumic acids, which are unique to oats among cereals. High-performance liquid chromatography allows a simultaneous quantification of free amino acids and biogenic amines in oat samples as their OPA/FMOC-CL (o-phthalaldehyde/9-fluorenylmethoxycarbonyl chloride) derivatives. In addition, an ultra-performance liquid chromatography/mass spectrometry method was developed to quantify and characterize avenanthramides contained in oat samples.

Keywords: Amino acids; Avenanthramides; Biogenic amines; HPLC; Oats; UPLC-MS.

MeSH terms

  • Amino Acids / analysis
  • Avena / chemistry*
  • Biogenic Amines / analysis
  • Chromatography* / methods
  • Chromatography, High Pressure Liquid / methods
  • Mass Spectrometry
  • Nutritive Value*
  • Plant Extracts / chemistry
  • ortho-Aminobenzoates / analysis


  • Amino Acids
  • Biogenic Amines
  • Plant Extracts
  • ortho-Aminobenzoates
  • avenanthramide-2C