Abstract
The primary features of Alzheimer's disease (AD) are extracellular amyloid plaques consisting mainly of deposits of amyloid β (Aβ) peptides and intracellular neurofibrillary tangles (NFTs). Sets of evidence suggest that interleukin-5 (IL-5) is involved in the pathogenesis of AD. Herein, we investigated the protective role of IL-5 in PC12 cells, to provide new insights into understanding this disease. Western blot was employed to assess the protein levels of Bax and phospho-tau as well as phospho-JAK2; MTT assay was performed to decipher cell viability. Treatment of IL-5 decreased Aβ25-35-induced tau phosphorylation and apoptosis, effects blunted by JAK2 inhibition. IL-5 prevents Aβ25-35-evoked tau protein hyperphosphorylation and apoptosis through JAK2 signaling.
Keywords:
Aβ25–35; IL-5; PC12 cells; Tau.
MeSH terms
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Alzheimer Disease / drug therapy
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Alzheimer Disease / metabolism
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Alzheimer Disease / pathology
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Amyloid beta-Peptides / metabolism*
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Animals
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Apoptosis* / drug effects
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Cell Survival / drug effects
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Enzyme Activation / drug effects
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Interleukin-5 / metabolism*
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Interleukin-5 Receptor alpha Subunit / agonists*
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Interleukin-5 Receptor alpha Subunit / antagonists & inhibitors
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Interleukin-5 Receptor alpha Subunit / genetics
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Interleukin-5 Receptor alpha Subunit / metabolism
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Janus Kinase 2 / antagonists & inhibitors
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Janus Kinase 2 / chemistry
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Janus Kinase 2 / metabolism
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Nerve Tissue Proteins / agonists
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Nerve Tissue Proteins / antagonists & inhibitors
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Nerve Tissue Proteins / genetics
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Nerve Tissue Proteins / metabolism
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Neurons / drug effects
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Neurons / metabolism*
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Neurons / pathology
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PC12 Cells
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Peptide Fragments / metabolism*
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Phosphorylation / drug effects
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Protein Kinase Inhibitors / pharmacology
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Protein Processing, Post-Translational* / drug effects
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Pyrrolidines / pharmacology
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RNA Interference
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Rats
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Signal Transduction / drug effects
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Sulfonamides / pharmacology
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tau Proteins / metabolism*
Substances
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Amyloid beta-Peptides
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Interleukin-5
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Interleukin-5 Receptor alpha Subunit
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Mapt protein, rat
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Nerve Tissue Proteins
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Peptide Fragments
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Protein Kinase Inhibitors
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Pyrrolidines
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Sulfonamides
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amyloid beta-protein (25-35)
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tau Proteins
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Fedratinib
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Jak2 protein, rat
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Janus Kinase 2