Abstract
We assessed the effect of sulforaphene (SFE) on osteoclast differentiation. SFE significantly decreased the number of RANKL-induced tartrate-resistant acid phosphatase-positive cells and suppressed pre-osteoclast multinucleation. Furthermore, SFE downregulated mRNA expression of DC-STAMP, OC-STAMP, and Atp6v0d2, which encode cell-cell fusion molecules. Our data suggest that SFE attenuates pre-osteoclast multinucleation via suppression of cell-cell fusion.
Keywords:
dendritic cell-specific transmembrane protein (DC-STAMP); isothiocyanate; osteoclast cell fusion; sulforaphene; v-ATPase V0 subunit d2(Atp6v0d2).
MeSH terms
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Animals
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Cell Differentiation / drug effects
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Cell Fusion
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Cell Line
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Cell Nucleus / drug effects
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Gene Expression Regulation
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Isothiocyanates / pharmacology*
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Macrophages / cytology
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Macrophages / drug effects*
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Macrophages / metabolism
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Membrane Proteins / antagonists & inhibitors*
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Membrane Proteins / genetics
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Membrane Proteins / metabolism
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Mice
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Nerve Tissue Proteins / antagonists & inhibitors*
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Nerve Tissue Proteins / genetics
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Nerve Tissue Proteins / metabolism
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Osteoclasts / cytology
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Osteoclasts / drug effects*
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Osteoclasts / metabolism
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RANK Ligand / pharmacology
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RNA, Messenger / antagonists & inhibitors
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Signal Transduction
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Vacuolar Proton-Translocating ATPases / antagonists & inhibitors*
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Vacuolar Proton-Translocating ATPases / genetics
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Vacuolar Proton-Translocating ATPases / metabolism
Substances
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DC-STAMP protein, mouse
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Isothiocyanates
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Membrane Proteins
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Nerve Tissue Proteins
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OC-STAMP protein, mouse
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RANK Ligand
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RNA, Messenger
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Tnfsf11 protein, mouse
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Atp6v0d2 protein, mouse
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Vacuolar Proton-Translocating ATPases
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sulphoraphene