Plant-derived pectin nanocoatings to prevent inflammatory cellular response of osteoblasts following Porphyromonas gingivalis infection

Int J Nanomedicine. 2017 Jan 12;12:433-445. doi: 10.2147/IJN.S113740. eCollection 2017.


Background: Bioengineered plant-derived Rhamnogalacturonan-Is (RG-Is) from pectins are potential candidates for surface nanocoating of medical devices. It has recently been reported that RG-I nanocoatings may prevent bacterial infection and improve the biocompatibility of implants. The aim of the study was to evaluate in vitro impact of bioengineered RG-I nanocoatings on osteogenic capacity and proinflammatory cytokine response of murine osteoblasts following Porphyromonas gingivalis infection.

Methods: Murine MC3T3-E1 osteoblasts and isolated primary calvarial osteoblasts from C57BL/6J (B6J osteoblasts) mice were infected with P. gingivalis and incubated on tissue culture polystyrene plates with or without nanocoatings of unmodified RG-Is isolated from potato pulps (PU) or dearabinanated RG-Is (PA). To investigate a behavior of infected osteoblasts cultured on RG-Is cell morphology, proliferation, metabolic activity, mineralization and osteogenic and pro-inflammatory gene expression were examined.

Results: Following P. gingivalis infection, PA, but not PU, significantly promoted MC3T3-E1 and BJ6 osteoblasts proliferation, metabolic activity, and calcium deposition. Moreover, Il-1b, Il-6, TNF-α, and Rankl gene expressions were downregulated in cells cultured on PU and to a higher extent on PA as compared to the corresponding control, whereas Runx, Alpl, Col1a1, and Bglap gene expressions were upregulated vice versa.

Conclusion: Our data clearly showed that pectin RG-Is nanocoating with high content of galactan (PA) reduces the osteoblastic response to P. gingivalis infection in vitro and may, therefore, reduce a risk of inflammation especially in immunocompromised patients with rheumatoid or periodontal disorders.

Keywords: Porphyromonas gingivalis; Rhamnogalacturonan-I; inflammation; nanocoatings; osteoblasts.

MeSH terms

  • Animals
  • Cell Proliferation / drug effects
  • Cell Shape / drug effects
  • Cells, Cultured
  • Coated Materials, Biocompatible / pharmacology*
  • Gene Expression Regulation / drug effects
  • Inflammation / pathology*
  • Mice, Inbred C57BL
  • Nanoparticles / chemistry*
  • Osteoblasts / drug effects
  • Osteoblasts / metabolism
  • Osteoblasts / microbiology*
  • Osteoblasts / pathology*
  • Pectins / pharmacology*
  • Porphyromonas gingivalis / drug effects
  • Porphyromonas gingivalis / physiology*
  • Real-Time Polymerase Chain Reaction
  • Solanum tuberosum / chemistry*


  • Coated Materials, Biocompatible
  • Pectins