Homeopathic medicine Cantharis modulates uropathogenic E. coli (UPEC)-induced cystitis in susceptible mice

Cytokine. 2017 Apr;92:103-109. doi: 10.1016/j.cyto.2017.01.014. Epub 2017 Jan 29.

Abstract

Objective: This is a random blinded placebo controlled murine experimental model to study the effects of Cantharis 6 CH, a homeopathic medicine, on E coli-induced cystitis.

Methods: 24 adult susceptible female BALB/c mice were inoculated with E coli - UPEC O4:K-:H5 by a transurethral catheter. Cantharis 6cH or vehicle (placebo) was offered to mice by free access into the drinking water (1:100), during 24 h after infection. Spleen, bladder and kidneys were processed for quantitative histopathology after immunohistochemistry, using anti-CD3, CD79, MIF, NK and VEGF antibodies; the cytokines present in the bladder washing fluid were measured using a LUMINEX-Magpix KIT. Mann-Whitney and Fisher exact test were used as statistical analysis.

Results: Cantharis 6 CH increased IL12p40, IFN-γ and decreased IL10 concentrations in the bladder fluid (p⩽0.05); in the bladder mucosa, it increased the ratio between B and T lymphocytes (31%) and between B lymphocytes and MIF+ macrophages (57%, p⩽0.05). In the pelvis, instead, it decreased the B/T cells ratio (41%, p⩽0.05) and increased the M1/M2 macrophage ratio (42%, p⩽0.05). No differences were seen in the kidney and spleen analysis.

Conclusion: The inverted balance of inflammatory cells and cytokines in bladder and pelvis mucosa shows specific local immune modulation induced by Cantharis 6cH.

Keywords: Cantharis; Cytokines; Escherichia coli; Homeopathy; UPEC.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cystitis / drug therapy*
  • Cystitis / immunology
  • Cystitis / microbiology
  • Cystitis / pathology
  • Cytokines / immunology
  • Escherichia coli Infections / drug therapy*
  • Escherichia coli Infections / immunology
  • Escherichia coli Infections / pathology
  • Female
  • Materia Medica / pharmacology*
  • Mice
  • Mice, Inbred BALB C
  • Urinary Tract Infections / drug therapy*
  • Urinary Tract Infections / immunology
  • Urinary Tract Infections / pathology
  • Uropathogenic Escherichia coli / immunology*

Substances

  • Cytokines
  • Materia Medica