Suspended Manufacture of Biological Structures

Adv Mater. 2017 Apr;29(13). doi: 10.1002/adma.201605594. Epub 2017 Feb 1.

Abstract

A method for the production of complex cell-laden structures is reported, which allows high-levels of spatial control over mechanical and chemical properties. The potential of this method for producing complicated tissues is demonstrated by manufacturing a complex hard/soft tissue interface and demonstrating that cell phenotype can be maintained over four weeks of culture.

Keywords: additive layer manufacturing; cartilage; hydrogels; osteochondral; tissue engineering.

MeSH terms

  • Cartilage / cytology
  • Cartilage / physiology
  • Cell Survival
  • Chondrocytes / cytology
  • Chondrocytes / physiology
  • Coculture Techniques
  • Computer Simulation
  • Elasticity
  • Femur / cytology
  • Femur / physiology
  • Humans
  • Hydrogels / chemistry
  • Materials Testing
  • Models, Biological
  • Osteoblasts / cytology
  • Osteoblasts / physiology
  • Polymers / chemistry
  • RNA, Messenger / metabolism
  • Rheology
  • Suspensions / chemistry
  • Tissue Engineering / methods*
  • Tissue Scaffolds*
  • Viscosity
  • X-Ray Microtomography

Substances

  • Hydrogels
  • Polymers
  • RNA, Messenger
  • Suspensions