Fluorescence and Bioluminescence Imaging of Orthotopic Brain Tumors in Mice

Methods Mol Biol. 2017;1530:283-305. doi: 10.1007/978-1-4939-6646-2_17.


Optical imaging strategies, such as fluorescence and bioluminescence imaging, are non-invasive, in vivo whole body imaging techniques utilized to study cancer. Optical imaging is widely used in preclinical work because of its ease of use and cost-friendliness. It also provides the opportunity to study animals and biological responses longitudinally over time. Important considerations include depth of tissue penetration, photon scattering, absorption and the choice of light emitting probe, all of which affect the resolution (image quality and data information) and the signal to noise ratio of the image. We describe how to use bioluminescence and fluorescence imaging to track a chemotherapeutic delivery nanocarrier conjugated with a fluorophore to determine its localization in vivo.

Keywords: Bioluminescence; Fluorescence; Glioblastoma; In vivo imaging; Optical imaging.

MeSH terms

  • Animals
  • Brain Neoplasms / diagnostic imaging*
  • Brain Neoplasms / pathology*
  • Cell Line, Tumor
  • Disease Models, Animal
  • Fluorescent Dyes
  • Gene Expression
  • Genes, Reporter
  • Image Processing, Computer-Assisted
  • Luminescent Measurements / methods*
  • Mice
  • Mice, Nude
  • Molecular Imaging / methods*
  • Optical Imaging / methods*
  • Tumor Burden


  • Fluorescent Dyes