Silencing myotubularin related protein 7 enhances proliferation and early differentiation of C2C12 myoblast

Biochem Biophys Res Commun. 2017 Mar 11;484(3):592-597. doi: 10.1016/j.bbrc.2017.01.143. Epub 2017 Jan 31.


Myotubularin related protein 7 (MTMR7) is a key member of the highly conserved myotubularin related proteins (MTMRs) family, which has phosphatase activity. MTMR7 was increased during myoblast differentiation and exhibited high expression level at primary fibers formation stages in pigs. This suggests that MTMR7 may be involved in myogenesis. In our study, we investigated the roles of MTMR7 on proliferation and differentiation of C2C12 myoblasts. Knocking down MTMR7 not only enhanced myoblast early differentiation via altering the expression of Myf5, but also promoted myoblast proliferation through increasing cyclinA2 expression. The improved proliferation capacity was related to the increased phosphorylation of AKT. Taken together, our research demonstrates that MTMR7 plays an important role in proliferation and early differentiation of C2C12 myoblast.

Keywords: C2C12 myoblast; Knockdown; MTMR7; Myogenesis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Differentiation / physiology
  • Cell Line
  • Cell Proliferation / physiology*
  • Cyclin A2 / metabolism*
  • Gene Silencing / physiology
  • Mice
  • Muscle Development / physiology*
  • Myoblasts / cytology*
  • Myoblasts / physiology*
  • Protein Tyrosine Phosphatases, Non-Receptor / genetics
  • Protein Tyrosine Phosphatases, Non-Receptor / metabolism*


  • CCNA2 protein, mouse
  • Cyclin A2
  • Mtmr7 protein, mouse
  • Protein Tyrosine Phosphatases, Non-Receptor