Immunohistochemical Detection of the Autophagy Markers LC3 and p62/SQSTM1 in Formalin-Fixed and Paraffin-Embedded Tissue

Sabina Berezowska; José A Galván

doi:10.1007/978-1-4939-6788-9_13

Immunohistochemical Detection of the Autophagy Markers LC3 and p62/SQSTM1 in Formalin-Fixed and Paraffin-Embedded Tissue

Methods Mol Biol. 2017:1560:189-194. doi: 10.1007/978-1-4939-6788-9_13.

Authors

Sabina Berezowska¹, José A Galván²

Affiliations

¹ Institute of Pathology, University of Bern, Murtenstrasse 31, 3010, Bern, Switzerland. sabina.berezowska@pathology.unibe.ch.
² Institute of Pathology, University of Bern, Murtenstrasse 31, 3010, Bern, Switzerland.

PMID: 28155154
DOI: 10.1007/978-1-4939-6788-9_13

Abstract

Autophagy is a highly conserved cellular mechanism of "self digestion," ensuring cellular homeostasis, and playing a role in many diseases including cancer. As a stress response mechanism, it may also be involved in cellular response to therapy.LC3 and Sequestosome 1 (p62/SQSTM1) are among the most widely used markers to monitor autophagy, and can be visualized in formalin-fixed and paraffin-embedded tissue by immunohistochemistry. Here we describe a validated staining protocol using an automated staining system available in many routine pathology laboratories, enabling high-throughput staining under standardized conditions.

Keywords: Autophagy; Immunohistochemistry; LC3; Sequestosome 1; p62/SQSTM1.

MeSH terms

Autophagy*
Biomarkers*
Humans
Immunohistochemistry* / methods
Microtubule-Associated Proteins / metabolism*
Neoplasms / diagnosis
Neoplasms / metabolism
Paraffin Embedding
Sequestosome-1 Protein / metabolism*

Substances

Biomarkers
MAP1LC3A protein, human
Microtubule-Associated Proteins
SQSTM1 protein, human
Sequestosome-1 Protein