Nile Red Staining of Neutral Lipids in Yeast

Kerry Ann Rostron; Clare Louise Lawrence

doi:10.1007/978-1-4939-6788-9_16

Nile Red Staining of Neutral Lipids in Yeast

Methods Mol Biol. 2017:1560:219-229. doi: 10.1007/978-1-4939-6788-9_16.

Authors

Kerry Ann Rostron^{1

2}, Clare Louise Lawrence³

Affiliations

¹ School of Pharmacy and Biomedical Sciences, University of Central Lancashire, Preston, Lancashire, UK.
² School of Biological Sciences, University of Reading, Reading, Berkshire, UK.
³ School of Pharmacy and Biomedical Sciences, University of Central Lancashire, Preston, Lancashire, PR1 2HE, UK. cllawrence@uclan.ac.uk.

PMID: 28155157
DOI: 10.1007/978-1-4939-6788-9_16

Abstract

Determination of cellular neutral lipid levels in yeast is important for both the biotechnology industry and biomedical research. However, many of the currently available methods are labor intensive and time consuming. Here we describe a rapid and repeatable method for the detection of neutral lipids, which can be utilized in both oleaginous and non-oleaginous yeast species. The method utilizes the fluorescent dye, Nile red, which enables neutral lipid levels to either be visualized via microscopy or quantified using a 96-well plate assay.

Keywords: Microplate; Microscopy; Neutral lipids; Nile red; Yeast.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Fluorescent Dyes*
Lipids* / chemistry
Microscopy, Fluorescence
Oxazines*
Staining and Labeling* / methods
Yeasts / metabolism*

Substances

Fluorescent Dyes
Lipids
Oxazines
nile red