High-Throughput Toxicity and Phenotypic Screening of 3D Human Neural Progenitor Cell Cultures on a Microarray Chip Platform

Stem Cell Reports. 2016 Nov 8;7(5):970-982. doi: 10.1016/j.stemcr.2016.10.001. Epub 2016 Oct 27.

Abstract

A 3D cell culture chip was used for high-throughput screening of a human neural progenitor cell line. The differential toxicity of 24 compounds was determined on undifferentiated and differentiating NPCs. Five compounds led to significant differences in IC50 values between undifferentiated and differentiating cultures. This platform has potential use in phenotypic screening to elucidate molecular toxicology on human stem cells.

Keywords: high-content screening; high-throughput microarray; human neural stem cells; neurotoxicity; screening; three-dimensional cell culture.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, N.I.H., Extramural

MeSH terms

  • Cell Culture Techniques
  • Cell Differentiation / drug effects
  • Cell Differentiation / genetics
  • Cell Survival / drug effects
  • Cell Survival / genetics
  • Dose-Response Relationship, Drug
  • Drug Discovery*
  • High-Throughput Screening Assays*
  • Humans
  • Neural Stem Cells / cytology
  • Neural Stem Cells / drug effects*
  • Neural Stem Cells / metabolism*
  • Oligonucleotide Array Sequence Analysis
  • Phenotype*
  • Toxicity Tests, Acute