Efficacy of vitamin B complex as an adjuvant therapy for the treatment of complicated vulvovaginal candidiasis: An in vivo and in vitro study

Biomed Pharmacother. 2017 Apr:88:770-777. doi: 10.1016/j.biopha.2017.01.001. Epub 2017 Jan 31.


Objective: This study aimed to explore the efficacy of vitamin B complex as an adjuvant therapy for the treatment of complicated vulvovaginal candidiasis (VVC) in vitro and in vivo.

Methods: One-hundred fifty-eight complicated VVC patients were randomly divided into group A (treated with suppository+oral antifungal agents), group B (treated with suppository+vaginal cream), and group C (treated with suppository+vaginal cream+oral vitamin B complex). A mouse model of VVC was established. Eighty VVC mice were randomly divided into 4 groups according to the dose of vitamin B complex (20 mice in each group): V1 group (injected with 150μL normal salin), V2 group (injected with 50μL vitamin B complex solution+100μL normal saline), V3 group (injected with 100μL vitamin B complex solution+50μL normal saline), and V4 group (injected with 150μL vitamin B complex solution). After 4 weeks of treatment, the vaginal secretion was obtained for microscopic smear examination. HE stainning was performed to observe histopathological changes of vaginal tissues. The expressions of inflammatory factors were detected by ELISA. Meanwhile, VVC model of vaginal epithelial cells was established. The effects of different concentrations of vitamin B complex on anti-fungal effect of fluconazole were detected in vitro.

Results: After the treatment, complicated patients in the group C had significantly higher effective rates than those in the group A and group B. After the intra-gastric administration, the microscopic smear examination found that obvious pseudohypha in cluster with a lot of blastospores can be seen in the vaginal secretions of mice in the V1 group under the microscope. There was significant difference between mice treated with different dosages of vitamin B complex. The inflammatory response of mice in the V1 group was significantly higher than those in other groups and the inflammation response reduced with the increase of vitamin B complex dosage. The vitamin B complex elevated the curative effects of fluconazole on VVC model of vaginal epithelial cells and significantly increased the anti-fungal effect of fluconazole.

Conclusions: Our findings suggest that vitamin B complex could be an effective adjuvant therapy for complicated VVC.

Keywords: Adjuvant therapy; Complicated vulvovaginal candidiasis; In vitro; In vivo; Vitamin B complex.

Publication types

  • Randomized Controlled Trial

MeSH terms

  • Adult
  • Animals
  • Antifungal Agents / administration & dosage
  • Antifungal Agents / therapeutic use
  • Candida / drug effects
  • Candidiasis, Vulvovaginal / drug therapy*
  • Candidiasis, Vulvovaginal / microbiology
  • Drug Therapy, Combination
  • Female
  • Fluconazole / pharmacology
  • Fluconazole / therapeutic use
  • Humans
  • Mice
  • Microbial Sensitivity Tests
  • Middle Aged
  • Ointments
  • Spores, Fungal / drug effects
  • Suppositories
  • Vitamin B Complex / administration & dosage
  • Vitamin B Complex / therapeutic use*
  • Vitamins / administration & dosage
  • Vitamins / therapeutic use*
  • Young Adult


  • Antifungal Agents
  • Ointments
  • Suppositories
  • Vitamins
  • Vitamin B Complex
  • Fluconazole