Functional properties of tropical banded cricket (Gryllodes sigillatus) protein hydrolysates

Food Chem. 2017 Jun 1;224:414-422. doi: 10.1016/j.foodchem.2016.11.138. Epub 2016 Nov 27.

Abstract

Recently, the benefits of entomophagy have been widely discussed. Due to western cultures' reluctance, entomophagy practices are leaning more towards incorporating insects into food products. In this study, whole crickets (Gryllodes sigillatus) were hydrolyzed with alcalase at 0.5, 1.5, and 3.0% (w/w) for 30, 60, and 90min. Degree of hydrolysis (DH), amino acid composition, solubility, emulsion and foaming properties were evaluated. Hydrolysis produced peptides with 26-52% DH compared to the control containing no enzyme (5% DH). Protein solubility of hydrolysates improved (p<0.05) over a range of pH's, exhibiting >30% soluble protein at pH 3 and 7 and 50-90% at alkaline pH, compared with the control. Emulsion activity index ranged from 7 to 32m2/g, while foamability ranged from 100 to 155% for all hydrolysates. These improved functional properties demonstrate the potential to develop cricket protein hydrolysates as a source of functional alternative protein in food ingredient formulations.

Keywords: Commercial enzyme; Cricket protein hydrolysates; Entomophagy; Functional properties.

MeSH terms

  • Amino Acids / analysis
  • Animals
  • Emulsions
  • Gryllidae / chemistry*
  • Hydrogen-Ion Concentration
  • Hydrolysis
  • Insect Proteins / chemistry*
  • Protein Hydrolysates / chemistry*
  • Solubility
  • Subtilisins / metabolism

Substances

  • Amino Acids
  • Emulsions
  • Insect Proteins
  • Protein Hydrolysates
  • Subtilisins