Humoral immune profiling of mycobacterial antigen recognition in sarcoidosis and Löfgren's syndrome using high-content peptide microarrays

Int J Infect Dis. 2017 Mar;56:167-175. doi: 10.1016/j.ijid.2017.01.021. Epub 2017 Jan 31.


Introduction: Sarcoidosis is considered an idiopathic granulomatous disease, although similar immunological and clinical features with tuberculosis (TB) suggest mycobacterial involvement in its pathogenesis. High-content peptide microarrays (HCPM) may help to decipher mycobacteria-specific antibody reactivity in sarcoidosis.

Methods: Serum samples from patients with sarcoidosis, Löfgren's syndrome, and TB, as well as from healthy individuals (12/group), were tested on HCPM containing 5964 individual peptides spanning 154 Mycobacterium tuberculosis proteins displayed as 15-amino acid stretches. Inclusion/exclusion and significance analyses were performed according to published methods.

Results: Each study group recognized 68-78% M. tuberculosis peptides at least once. M. tuberculosis epitope recognition by sarcoidosis patient sera was 42.7%, and by TB patient sera was 39.1%. Seven and 16 peptides were recognized in 9/12 (75%) and 8/12 (67%) sarcoidosis patient sera but not in TB patient sera, respectively. Nine (75%) and eight (67%) out of twelve TB patient sera, respectively recognized M. tuberculosis peptides that were not recognized in sarcoidosis patient sera.

Conclusions: Specific IgG recognition patterns for M. tuberculosis antigens in sarcoidosis patients re-affirm mycobacterial involvement in sarcoidosis, providing biologically relevant targets for future studies pertaining to diagnostics and immunotherapy.

Keywords: Humoral immune responses; Löfgren’s syndrome; Peptide microarray; Sarcoidosis; Tuberculosis; tuberculosis antigens.

MeSH terms

  • Antibody Formation / immunology*
  • Antigens, Bacterial / immunology*
  • Bacterial Proteins / immunology
  • Female
  • Humans
  • Immunity, Humoral / immunology*
  • Male
  • Mycobacterium tuberculosis / immunology*
  • Peptides / immunology
  • Protein Array Analysis / methods*
  • Sarcoidosis / blood
  • Sarcoidosis / immunology*
  • Syndrome


  • Antigens, Bacterial
  • Bacterial Proteins
  • Peptides