Mammalian verprolin CR16 acts as a modulator of ITSN scaffold proteins association with actin

Biochem Biophys Res Commun. 2017 Mar 18;484(4):813-819. doi: 10.1016/j.bbrc.2017.01.177. Epub 2017 Feb 1.

Abstract

Actin cytoskeleton rearrangements are required for normal cell functioning, and their deregulation leads to various pathologies. Members of two mammalian protein families - ITSNs (ITSN1 and ITSN2) and verprolins (WIP, CR16 and WIRE) are involved in Cdc42/N-WASP/Arp2/3 signaling pathway-mediated remodeling of the actin cytoskeleton. Recently we demonstrated that ITSNs interact with the actin-regulating protein WIP. Here, we show that other member of verprolin family, CR16, also forms complexes with ITSN1 and ITSN2 in human cell lines. The actin-binding protein CR16 modulates ITSN/β-actin association. Moreover, overexpressed CR16 promoted co-localization of ITSN1 with F-actin in MCF-7 breast cancer cells. Our data demonstrated that CR16 mRNA is expressed in glioblastoma and breast tumors. These findings provide the basis for further functional investigations of the ITSN/CR16 complex that may play an important role in actin remodeling and cellular invasion.

Keywords: Actin cytoskeleton; CR16; ITSN; Scaffold protein.

MeSH terms

  • Actin Cytoskeleton / metabolism*
  • Actins / metabolism*
  • Adaptor Proteins, Vesicular Transport / metabolism*
  • Cytoskeletal Proteins / metabolism*
  • Humans
  • MCF-7 Cells
  • Microfilament Proteins / metabolism*
  • Protein Binding

Substances

  • Actins
  • Adaptor Proteins, Vesicular Transport
  • Cytoskeletal Proteins
  • ITSN1 protein, human
  • ITSN2 protein, human
  • Microfilament Proteins
  • WIPF3 protein, human