Behavior of Gingival Fibroblasts on Titanium Implant Surfaces in Combination with either Injectable-PRF or PRP

Int J Mol Sci. 2017 Feb 4;18(2):331. doi: 10.3390/ijms18020331.


Various strategies have been employed to speed tissue regeneration using bioactive molecules. Interestingly, platelet concentrates derived from a patient's own blood have been utilized as a regenerative strategy in recent years. In the present study, a novel liquid platelet formulation prepared without the use of anti-coagulants (injectable-platelet-rich fibrin, i-PRF) was compared to standard platelet-rich plasma (PRP) with gingival fibroblasts cultured on smooth and roughened titanium implant surfaces. Standard PRP and i-PRF (centrifuged at 700 rpm (60× g) for 3 min) were compared by assays for fibroblast biocompatibility, migration, adhesion, proliferation, as well as expression of platelet-derived growth factor (PDGF), transforming growth factor-β (TGF-β), collagen1 (COL1) and fibronectin (FN). The results demonstrate that i-PRF induced significantly higher cell migration, as well as higher messenger RNA (mRNA) levels of PDGF, TGF-β, collagen1 and fibronectin when compared to PRP. Furthermore, collagen1 synthesis was highest in the i-PRF group. These findings demonstrate that liquid platelet concentrates can be formulated without the use of anticoagulants and present much translational potential for future research. Future animal and clinical trials are now necessary to further investigate the potential of utilizing i-PRF for soft tissue regenerative protocols in combination with various biomaterials.

Keywords: blood; fibrin; fibroblasts; platelet-rich fibrin; platelets; regeneration; wound healing.

MeSH terms

  • Cell Adhesion
  • Cell Movement
  • Cell Proliferation
  • Cell Survival
  • Collagen Type I / metabolism
  • Dental Implants*
  • Extracellular Matrix Proteins / genetics
  • Extracellular Matrix Proteins / metabolism
  • Fibrin / metabolism
  • Fibroblasts / physiology*
  • Gingiva / cytology*
  • Humans
  • Platelet-Rich Plasma*
  • Regeneration / genetics
  • Surface Properties
  • Titanium* / chemistry
  • Wound Healing


  • Collagen Type I
  • Dental Implants
  • Extracellular Matrix Proteins
  • Fibrin
  • Titanium