Robust, universal biomarker assay to detect senescent cells in biological specimens

Aging Cell. 2017 Feb;16(1):192-197. doi: 10.1111/acel.12545. Epub 2016 Nov 17.


Cellular senescence contributes to organismal development, aging, and diverse pathologies, yet available assays to detect senescent cells remain unsatisfactory. Here, we designed and synthesized a lipophilic, biotin-linked Sudan Black B (SBB) analogue suitable for sensitive and specific, antibody-enhanced detection of lipofuscin-containing senescent cells in any biological material. This new hybrid histo-/immunochemical method is easy to perform, reliable, and universally applicable to assess senescence in biomedicine, from cancer research to gerontology.

Keywords: Sudan Black B; aging; biotin‐linked compounds; immunohistochemistry; senescence.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biological Assay / methods*
  • Biomarkers / analysis*
  • Biotin / metabolism
  • Cell Line, Tumor
  • Cellular Senescence*
  • Humans
  • Mice


  • Biomarkers
  • Biotin