Transition from a maternal to external nitrogen source in maize seedlings

Abstract

Maximizing NO₃^- uptake during seedling development is important as it has a major influence on plant growth and yield. However, little is known about the processes leading to, and involved in, the initiation of root NO₃^- uptake capacity in developing seedlings. This study examines the physiological processes involved in root NO₃^- uptake and metabolism, to gain an understanding of how the NO₃^- uptake system responds to meet demand as maize seedlings transition from seed N use to external N capture. The concentrations of seed-derived free amino acids within root and shoot tissues are initially high, but decrease rapidly until stabilizing eight days after imbibition (DAI). Similarly, shoot N% decreases, but does not stabilize until 12-13 DAI. Following the decrease in free amino acid concentrations, root NO₃^- uptake capacity increases until shoot N% stabilizes. The increase in root NO₃^- uptake capacity corresponds with a rapid rise in transcript levels of putative NO₃^- transporters, ZmNRT2.1 and ZmNRT2.2. The processes underlying the increase in root NO₃^- uptake capacity to meet N demand provide an insight into the processes controlling N uptake.