Hypomethylation of intron1 of α-synuclein gene does not correlate with Parkinson's disease

Mol Brain. 2017 Feb 7;10(1):6. doi: 10.1186/s13041-017-0285-z.


Deregulation of α-synuclein encoding gene (SNCA) is one of the important facets of Parkinson's disease (PD) research. DNA methylation status of SNCA-intron1 has been shown to regulate the α-synuclein expression. The present study is aimed at investigating whether methylation of SNCA-intron1 is associated with higher expression of α-synuclein in PD. We have investigated the intron1 methylation status from 16 post-mortem brain samples comprised of 8 PD and 8 control subjects using bisulfite sequencing. We further correlated this methylation status with α-synuclein protein levels in substantia nigra of that individual using western blot analysis. We did not observe any significant difference in methylation of SNCA-intron1 region between PD and control samples. Moreover, no correlation was observed between methylation of SNCA-intron1 with α-synuclein level. Methylation of SNCA-intron1 region does not correlate with α-synuclein expression in PD samples.

Keywords: DNA methylation; Epigenetic regulation; Parkinson’s Disease; α-synuclein.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, N.I.H., Extramural

MeSH terms

  • Aged
  • Aged, 80 and over
  • Base Sequence
  • Case-Control Studies
  • DNA Methylation / genetics*
  • Humans
  • Introns / genetics*
  • Middle Aged
  • Parkinson Disease / genetics*
  • alpha-Synuclein / genetics*


  • alpha-Synuclein