Physicochemical characterization of chitosan-hyaluronan-coated solid lipid nanoparticles for the targeted delivery of paclitaxel: a proof-of-concept study in breast cancer cells

Nanomedicine (Lond). 2017 Mar;12(5):473-490. doi: 10.2217/nnm-2016-0371. Epub 2017 Feb 9.

Abstract

Aim: To investigate the potential of modified solid lipid nanoparticles (SLN) for the delivery of paclitaxel (PAX).

Materials & methods: SLN loaded with PAX were prepared via modified high-pressure hot homogenization. Formulation parameters were optimized to obtain a high-quality delivery system. SLN cores were coated, layer-by-layer, with a chitosan and hyaluronan (HA) shell. Selectivity toward HA receptors was tested in a breast cancer cell line, MCF-7.

Results: Stable and reproducible nano-sized and negatively charged nanoparticles resulted. Findings reveal that chitosan-HA-coated SLN facilitated the targeting, cellular uptake and the time-/dose-controlled delivery and release of PAX, enhancing intrinsic chemotherapeutic activities.

Conclusion: SLN are suitable carrier candidates for nano-oncology given their localized, and potent cytotoxic potential overcoming multidrug-resistant cancer cells.

Keywords: chitosan; drug delivery; hyaluronan; layer-by-layer; multidrug resistance; nanoncology; paclitaxel; solid lipid nanoparticles.

MeSH terms

  • Breast Neoplasms / drug therapy*
  • Breast Neoplasms / pathology
  • Cell Survival / drug effects
  • Chitosan / chemistry
  • Drug Delivery Systems*
  • Drug Stability
  • Female
  • Humans
  • Hyaluronic Acid / chemistry
  • Lipids / administration & dosage
  • Lipids / chemistry
  • MCF-7 Cells
  • Nanoparticles / administration & dosage*
  • Nanoparticles / chemistry
  • Paclitaxel / administration & dosage*
  • Paclitaxel / chemistry

Substances

  • Lipids
  • Hyaluronic Acid
  • Chitosan
  • Paclitaxel