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. 2017 Mar;12(5):491-510.
doi: 10.2217/nnm-2016-0295. Epub 2017 Feb 9.

Controlled Release of Antigen and Toll-like Receptor Ligands From PLGA Nanoparticles Enhances Immunogenicity

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Controlled Release of Antigen and Toll-like Receptor Ligands From PLGA Nanoparticles Enhances Immunogenicity

Luis J Cruz et al. Nanomedicine (Lond). .

Abstract

Aim: Dendritic cells rapidly capture nanoparticles and induce a potent cellular immune response. It is yet unknown whether the immunological response induced by slow release of encapsulated versus soluble antigen and adjuvant is superior.

Materials & methods: The kinetics of poly(lactic-co-glycolic acid) PLGA nanoparticles antigen release was studied by the DQ-bovine serum albumin (BSA) self-quenching antigen model. The immunological response induced was evaluated by means of dendritic cell activation/maturation markers, cytokine production and their ability to drive antigen-specific T-cell proliferation.

Results & conclusion: PLGA-encapsulated antigen and adjuvant showed an enhanced T-cell response when compared with soluble vaccine components by increasing antigenicity and adjuvanticity. Although the kinetic profile followed the same pattern, encapsulation increased strength and duration of the response.

Keywords: biocompatible materials; dendritic cells; fluorescence; nanoparticles.

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