Changes in protein expression after treatment with Ancylostoma caninum excretory/secretory products in a mouse model of colitis

Sci Rep. 2017 Feb 13;7:41883. doi: 10.1038/srep41883.

Abstract

Different reports have highlighted the potential use of helminths and their secretions in the treatment of inflammatory bowel disease (IBD) conditions; however, no reports have investigated their effects at a proteome level. Herein, we characterise the protein expression changes that occur in lamina propria (LP) and the intestinal epithelial cells (IEC) of mice with dextran sulfate sodium (DSS)-induced colitis treated with Ancylostoma caninum excretory/secretory (ES) products using a quantitative proteomic approach. We have shown how parasite products can significantly alter the expression of proteins involved in immune responses, cell death and with an antioxidant activity. Interestingly, significant changes in the expression levels of different mucins were observed in this study. MUC13, a mucin implicated in gastrointestinal homeostasis, was upregulated in the LP of mice with DSS-induced colitis treated with ES, while MUC2, a major component of mucus, was upregulated in the IEC. In addition, A. caninum proteins have an important effect on proteins with antioxidant functions and proteins involved in intestinal homeostasis and tissue integrity and regeneration. Understanding how parasites can ameliorate IBD pathogenesis can help us design novel treatments for autoimmune diseases.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Ancylostoma / chemistry*
  • Ancylostoma / metabolism
  • Animals
  • Anti-Inflammatory Agents / pharmacology*
  • Anti-Inflammatory Agents / therapeutic use
  • Antigens, Surface / genetics
  • Antigens, Surface / metabolism
  • Biological Products / pharmacology*
  • Biological Products / therapeutic use
  • Colitis, Ulcerative / drug therapy*
  • Colitis, Ulcerative / etiology
  • Dextran Sulfate / toxicity
  • Epidermal Growth Factor / genetics
  • Epidermal Growth Factor / metabolism
  • Female
  • Helminth Proteins / pharmacology*
  • Helminth Proteins / therapeutic use
  • Intestinal Mucosa / drug effects*
  • Intestinal Mucosa / metabolism
  • Mice
  • Mice, Inbred C57BL
  • Mucin-2 / genetics
  • Mucin-2 / metabolism
  • Up-Regulation

Substances

  • Anti-Inflammatory Agents
  • Antigens, Surface
  • Biological Products
  • Helminth Proteins
  • Ly64 protein, mouse
  • Muc2 protein, mouse
  • Mucin-2
  • Epidermal Growth Factor
  • Dextran Sulfate