Biosensing of DNA oxidative damage: a model of using glucose meter for non-glucose biomarker detection

Int J Nanomedicine. 2017 Feb 2;12:979-987. doi: 10.2147/IJN.S125437. eCollection 2017.

Abstract

Non-glucose biomarker-DNA oxidative damage biomarker 8-hydroxy-2'-deoxyguanosine (8-OHdG) has been successfully detected using a smartphone-enabled glucose meter. Through a series of immune reactions and enzymatic reactions on a solid lateral flow platform, 8-OHdG concentration has been converted to a relative amount of glucose, and therefore can be detected by conventional glucose meter directly. The device was able to detect 8-OHdG concentrations in phosphate buffer saline as low as 1.73 ng mL-1 with a dynamic range of 1-200 ng mL-1. Considering the inherent advantages of the personal glucose meter, the demonstration of this device, therefore, should provide new opportunities for the monitoring of a wide range of biomarkers and various target analytes in connection with different molecular recognition events.

Keywords: 8-OHdG; DNA oxidative damage; POCTs; biosensor; immunostrip; point-of-care.

MeSH terms

  • 8-Hydroxy-2'-Deoxyguanosine
  • Biomarkers / analysis
  • Biosensing Techniques / instrumentation
  • Biosensing Techniques / methods*
  • Blood Glucose Self-Monitoring / instrumentation*
  • DNA Damage*
  • Deoxyguanosine / analogs & derivatives*
  • Deoxyguanosine / analysis
  • Equipment Design
  • Glucose / metabolism
  • Guanosine / analogs & derivatives
  • Guanosine / chemistry
  • Oxidation-Reduction
  • Smartphone
  • beta-Fructofuranosidase / chemistry
  • beta-Fructofuranosidase / metabolism

Substances

  • Biomarkers
  • Guanosine
  • 8-hydroxyguanosine
  • 8-Hydroxy-2'-Deoxyguanosine
  • beta-Fructofuranosidase
  • Deoxyguanosine
  • Glucose