Application of a Novel CD206+ Macrophage-Specific Arterial Imaging Strategy in HIV-Infected Individuals

J Infect Dis. 2017 Apr 15;215(8):1264-1269. doi: 10.1093/infdis/jix095.


Background: The ability to noninvasively assess arterial CD206+ macrophages may lead to improved understanding of human immunodeficiency virus (HIV)-associated cardiovascular disease.

Methods: We trialed a novel macrophage-specific arterial imaging technique.

Results: We demonstrated colocalization between technetium Tc 99m tilmanocept (99mTc-tilmanocept) and CD206+ macrophages ex vivo. In vivo application of 99mTc-tilmanocept single-photon emission computed tomography/computed tomography revealed high-level 99mTc-tilmanocept uptake across 20.4% of the aortic surface volume among HIV-infected subjects, compared with 4.3% among non-HIV-infected subjects (P = .009). Among all subjects, aortic high-level 99mTc-tilmanocept uptake was related to noncalcified aortic plaque volume (r = 0.87; P = .003) on computed tomographic angiography, and this relationship held when we controlled for HIV status.

Conclusion: These first-in-human data introduce a novel macrophage-specific arterial imaging technique in HIV.

Clinical trials registration: NCT02542371.

Keywords: HIV-associated cardiovascular disease; arterial inflammation; atherosclerosis..

Publication types

  • Observational Study

MeSH terms

  • Aorta / diagnostic imaging
  • Atherosclerosis / diagnostic imaging*
  • Atherosclerosis / etiology
  • Case-Control Studies
  • Cross-Sectional Studies
  • Dextrans
  • HIV Infections / complications*
  • Humans
  • Lectins, C-Type / metabolism
  • Lymph Nodes / diagnostic imaging
  • Macrophages / cytology*
  • Male
  • Mannans
  • Mannose Receptor
  • Mannose-Binding Lectins / metabolism
  • Middle Aged
  • Plaque, Atherosclerotic / diagnostic imaging*
  • Plaque, Atherosclerotic / virology
  • Radiopharmaceuticals
  • Receptors, Cell Surface / metabolism
  • Regression Analysis
  • Single Photon Emission Computed Tomography Computed Tomography
  • Technetium Tc 99m Pentetate / analogs & derivatives
  • United States


  • Dextrans
  • Lectins, C-Type
  • Mannans
  • Mannose Receptor
  • Mannose-Binding Lectins
  • Radiopharmaceuticals
  • Receptors, Cell Surface
  • technetium-diethylenetriaminepentaacetic acid-mannosyl-dextran
  • Technetium Tc 99m Pentetate

Associated data