Chitosan-Recombinamer Layer-by-Layer Coatings for Multifunctional Implants

Int J Mol Sci. 2017 Feb 9;18(2):369. doi: 10.3390/ijms18020369.


The main clinical problems for dental implants are (1) formation of biofilm around the implant-a condition known as peri-implantitis and (2) inadequate bone formation around the implant-lack of osseointegration. Therefore, developing an implant to overcome these problems is of significant interest to the dental community. Chitosan has been reported to have good biocompatibility and anti-bacterial activity. An osseo-inductive recombinant elastin-like biopolymer (P-HAP), that contains a peptide derived from the protein statherin, has been reported to induce biomineralization and osteoblast differentiation. In this study, chitosan/P-HAP bi-layers were built on a titanium surface using a layer-by-layer (LbL) assembly technique. The difference in the water contact angle between consecutive layers, the representative peaks in diffuse reflectance infrared Fourier transform spectroscopy (DRIFTS), X-ray photoelectron spectroscopy (XPS), and the changes in the topography between surfaces with a different number of bi-layers observed using atomic force microscopy (AFM), all indicated the successful establishment of chitosan/P-HAP LbL assembly on the titanium surface. The LbL-modified surfaces showed increased biomineralization, an appropriate mouse pre-osteoblastic cell response, and significant anti-bacterial activity against Streptococcus gordonii, a primary colonizer of tissues in the oral environment.

Keywords: chitosan; elastin-like recombinamers; implant; layer-by-layer; titanium.

MeSH terms

  • Anti-Bacterial Agents / chemistry
  • Cell Adhesion
  • Chitosan / chemistry*
  • Coated Materials, Biocompatible / chemistry*
  • Dental Implants
  • Microscopy, Atomic Force
  • Osseointegration
  • Osteoblasts / metabolism
  • Photoelectron Spectroscopy
  • Prostheses and Implants*
  • Proteins / chemistry
  • Spectroscopy, Fourier Transform Infrared
  • Surface Properties
  • Titanium / chemistry


  • Anti-Bacterial Agents
  • Coated Materials, Biocompatible
  • Dental Implants
  • Proteins
  • Chitosan
  • Titanium