Transcriptional and Cell Cycle Alterations Mark Aging of Primary Human Adipose-Derived Stem Cells

Stem Cells. 2017 May;35(5):1392-1401. doi: 10.1002/stem.2592. Epub 2017 Mar 5.


Adult stem cells play a critical role in the maintenance of tissue homeostasis and prevention of aging. While the regenerative potential of stem cells with low cellular turnover, such as adipose-derived stem cells (ASCs), is increasingly recognized, the study of chronological aging in ASCs is technically difficult and remains poorly understood. Here, we use our model of chronological aging in primary human ASCs to examine genome-wide transcriptional networks. We demonstrate first that the transcriptome of aging ASCs is distinctly more stable than that of age-matched fibroblasts, and further, that age-dependent modifications in cell cycle progression and translation initiation specifically characterize aging ASCs in conjunction with increased nascent protein synthesis and a distinctly shortened G1 phase. Our results reveal novel chronological aging mechanisms in ASCs that are inherently different from differentiated cells and that may reflect an organismal attempt to meet the increased demands of tissue and organ homeostasis during aging. Stem Cells 2017;35:1392-1401.

Keywords: Adipose-derived stem cell; Aging; Cell cycle; Human adipose-derived stem cell; Transcriptome; Translation initiation.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Adipose Tissue / cytology*
  • Adult
  • Cell Cycle* / genetics
  • Cells, Cultured
  • Cellular Senescence* / genetics
  • Female
  • Fibroblasts / cytology
  • Fibroblasts / metabolism
  • G1 Phase / genetics
  • Gene Expression Profiling
  • Gene Expression Regulation
  • Humans
  • Middle Aged
  • Mitosis / genetics
  • Models, Biological
  • Protein Biosynthesis / genetics
  • Protein Processing, Post-Translational / genetics
  • Stem Cells / cytology*
  • Transcription, Genetic*
  • Transcriptome / genetics
  • Young Adult