Reconstitution of a functional interleukin 2 receptor in a nonlymphoid cell

J Immunol. 1987 Oct 1;139(7):2355-60.


The maintenance of T lymphocytes which are important effectors of immune responses requires the T cell growth factor, interleukin 2 (IL-2). The binding of IL-2 to specific cell-surface receptors (IL-2R) has previously been shown to be essential to the growth and proliferation of activated lymphocytes. A human IL-2R cDNA sequence, placed under the control of the SV40 transcriptional promoter and enhancer, has been transfected into murine L cells. Single cell analysis by autoradiography was used to show that fibroblastic L cells, stably expressing human IL-2R, respond to stimulation with IL-2 by DNA synthesis and proliferation. This response is specifically blocked by the addition of an anti-IL-2R monoclonal antibody, anti-Tac, as previously reported. Neither nonspecific antisera nor 7G7/B6, an anti-IL-2R monoclonal antibody which does not interfere with IL-2 binding to its receptor, had any effect on this response. The induction of DNA synthesis by IL-2 is both rapid and dose-dependent. The ability of IL-2 to stimulate these transfected L cells to proliferate demonstrates that a lymphoid environment is not required for the functional interaction between IL-2 and its receptor, and provides a unique model system for the investigation of the molecular basis for the cellular events mediated by IL-2.

MeSH terms

  • Animals
  • Antigens, Surface / genetics
  • Cell Division / drug effects
  • DNA / genetics
  • DNA Replication / drug effects
  • Humans
  • Interleukin-2 / metabolism*
  • Interleukin-2 / pharmacology
  • L Cells / drug effects
  • L Cells / metabolism
  • Mice
  • Receptors, Immunologic / genetics
  • Receptors, Immunologic / metabolism*
  • Receptors, Interleukin-2
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism*
  • Recombinant Proteins / metabolism*
  • Tumor Necrosis Factor Receptor Superfamily, Member 7


  • Antigens, Surface
  • Interleukin-2
  • Receptors, Immunologic
  • Receptors, Interleukin-2
  • Recombinant Fusion Proteins
  • Recombinant Proteins
  • Tumor Necrosis Factor Receptor Superfamily, Member 7
  • DNA