Phytoremediation of dyes using Lagerstroemia speciosa mediated silver nanoparticles and its biofilm activity against clinical strains Pseudomonas aeruginosa

J Photochem Photobiol B. 2017 Mar:168:107-116. doi: 10.1016/j.jphotobiol.2017.02.004. Epub 2017 Feb 10.

Abstract

The aim of this study was to prepare silver nanoparticles by a green method using the aqueous leaves extract of Lagerstroemia speciosa. The prepared silver nanoparticles were characterized, studied for its photocatalytic and biofilm inhibition studies. The maximum absorbance peak was found at 427nm and thus confirming the formation of silver nanoparticles. The average size of silver nanoparticles synthesized was found to be 12nm using XRD and it was spherical in shape. The nanoparticles synthesized was investigated for photocatalytic activity for to two different dye molecules, methyl orange and methylene blue showing 310 and 290min degradation time respectively. The silver nanoparticles biofilm inhibition assay against clinical strains of Pseudomonas aeruginosa showed lowest accumulation at a lower concentration. The biofilm inhibition was also studied by visual interpretation through Scanning Electron Microscopy states that 50μgmL-1 exerts the highest inhibition compared against the control. This evident helps to analysis the silver nanoparticles for various applications in future.

Keywords: Antibiofilm activity; Dye degradation; Lagerstroemia speciosa; Methyl orange; Methylene blue; Scanning electron microscopy; Silver nanoparticle.

MeSH terms

  • Anti-Bacterial Agents / isolation & purification
  • Anti-Bacterial Agents / pharmacology
  • Biodegradation, Environmental*
  • Biofilms / drug effects*
  • Coloring Agents / metabolism*
  • Lagerstroemia
  • Metal Nanoparticles / chemistry*
  • Plant Extracts / pharmacology*
  • Pseudomonas aeruginosa / drug effects
  • Silver

Substances

  • Anti-Bacterial Agents
  • Coloring Agents
  • Plant Extracts
  • Silver