Histidine proton resonances of carbonmonoxyhaemoglobins A and Cowtown in chloride-free buffer

J Mol Biol. 1987 May 20;195(2):453-5. doi: 10.1016/0022-2836(87)90666-8.


A re-examination of the C-2 histidine proton resonances of haemoglobins A and Cowtown (His HC3(146) beta----Leu) in chloride-free Hepes buffer has shown that all the resonances present in haemoglobin A are present in haemoglobin Cowtown, so that the pKa of His HC3(146) beta cannot be determined by nuclear magnetic resonance in this buffer.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Carboxyhemoglobin*
  • Hemoglobin A*
  • Hemoglobins, Abnormal*
  • Histidine*
  • Humans
  • Magnetic Resonance Spectroscopy
  • Protons*


  • Hemoglobins, Abnormal
  • Protons
  • Histidine
  • hemoglobin Cowtown
  • Hemoglobin A
  • Carboxyhemoglobin