Preparation and biocompatibility of a chitin nanofiber/gelatin composite film

Int J Biol Macromol. 2017 Nov;104(Pt B):1882-1889. doi: 10.1016/j.ijbiomac.2017.02.041. Epub 2017 Feb 16.


The development of chitin-based materials with favorable mechanical properties and biocompatibility is an important research goal owing to the wide-ranging practical applications. In this study, a composite film was prepared using chitin nanofibers and gelatin. The CNF/gelatin composite film was highly viscous and had a fine nanofiber structure. The transmittances indicated high transparency, regardless of nanofiber content. The water content of the CNF/gelatin composite film increased linearly as the gelatin content increased. Although the CNF/gelatin composite film did not induce severe inflammation, it strongly induced fibroblast proliferation, indicating high biocompatibility. Based on these results, the films are suitable for biological applications, e.g., tissue engineering, medicines, and cosmetics.

Keywords: Chitin nanofiber; Composite; Gelatin.

MeSH terms

  • Animals
  • Biocompatible Materials / chemistry*
  • Chitin / chemistry*
  • Female
  • Gelatin / chemistry*
  • Gelatin / ultrastructure
  • Materials Testing
  • Mechanical Phenomena
  • Mice
  • Nanofibers / chemistry*
  • Surface Properties


  • Biocompatible Materials
  • gelatin film
  • Chitin
  • Gelatin