Use of human methylation arrays for epigenome research in the common marmoset (Callithrix jacchus)

Neurosci Res. 2017 Jul;120:60-65. doi: 10.1016/j.neures.2017.02.005. Epub 2017 Feb 17.

Abstract

We examined the usefulness of commercially available DNA methylation arrays designed for the human genome (Illumina HumanMethylation450 and MethylationEPIC) for high-throughput epigenome analysis of the common marmoset, a nonhuman primate suitable for research on neuropsychiatric disorders. From among the probes on the methylation arrays, we selected those available for the common marmoset. DNA methylation data were obtained from genomic DNA extracted from the frontal cortex and blood samples of adult common marmosets as well as the frontal cortex of neonatal marmosets. About 10% of the probes on the arrays were estimated to be useful for DNA methylation assay in the common marmoset. Strong correlations existed between human and marmoset DNA methylation data. Illumina methylation arrays are useful for epigenome research using the common marmoset.

Keywords: Beadarray; Cytosine modification; DNA methylation; Epigenetics; Nonhuman primate.

MeSH terms

  • Animals
  • Callithrix
  • DNA Methylation*
  • Epigenesis, Genetic*
  • Epigenomics / instrumentation
  • Epigenomics / methods*
  • Humans
  • Male
  • Species Specificity