Lipidomics, en route to accurate quantitation

Biochim Biophys Acta Mol Cell Biol Lipids. 2017 Aug;1862(8):752-761. doi: 10.1016/j.bbalip.2017.02.008. Epub 2017 Feb 16.


Accurate quantitation is prerequisite for the sustainable development of lipidomics via enabling its applications in various biological and biomedical settings. In this review, the technical considerations and limitations of existent lipidomics technologies, particularly in terms of accurate quantitation; as well as the potential sources of errors along a typical lipidomic workflow that could ultimately give rise to quantitative inaccuracies will be addressed. Furthermore, the pressing need to exercise stricter definitions of terms and protocol standardization pertaining to quantitative lipidomics will be critically discussed, as quantitative accuracy may substantially impact upon the persevering development of lipidomics in the long run. This article is part of a Special Issue entitled: BBALIP_Lipidomics Opinion Articles edited by Sepp Kohlwein.

Keywords: Accurate quantitation; Internal standards; Lipid extraction; Lipidomics.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Data Accuracy
  • Evaluation Studies as Topic
  • Humans
  • Lipid Metabolism / physiology*
  • Lipids / chemistry*
  • Metabolomics / methods
  • Reference Standards
  • Technology / methods
  • Workflow


  • Lipids