Detecting RNA modifications in the epitranscriptome: predict and validate

Nat Rev Genet. 2017 May;18(5):275-291. doi: 10.1038/nrg.2016.169. Epub 2017 Feb 20.


RNA modifications are emerging players in the field of post-transcriptional regulation of gene expression, and are attracting a comparable degree of research interest to DNA and histone modifications in the field of epigenetics. We now know of more than 150 RNA modifications and the true potential of a few of these is currently emerging as the consequence of a leap in detection technology, principally associated with high-throughput sequencing. This Review outlines the major developments in this field through a structured discussion of detection principles, lays out advantages and drawbacks of new high-throughput methods and presents conventional biophysical identification of modifications as meaningful ways for validation.

Publication types

  • Review
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Epigenesis, Genetic / genetics*
  • Epigenomics / methods*
  • Gene Expression Regulation
  • Humans
  • RNA / chemistry*
  • RNA / genetics
  • RNA / metabolism*
  • RNA Processing, Post-Transcriptional*
  • Transcriptome / genetics*


  • RNA