Cell-matrix signals specify bone endothelial cells during developmental osteogenesis

Nat Cell Biol. 2017 Mar;19(3):189-201. doi: 10.1038/ncb3476. Epub 2017 Feb 20.


Blood vessels in the mammalian skeletal system control bone formation and support haematopoiesis by generating local niche environments. While a specialized capillary subtype, termed type H, has been recently shown to couple angiogenesis and osteogenesis in adolescent, adult and ageing mice, little is known about the formation of specific endothelial cell populations during early developmental endochondral bone formation. Here, we report that embryonic and early postnatal long bone contains a specialized endothelial cell subtype, termed type E, which strongly supports osteoblast lineage cells and later gives rise to other endothelial cell subpopulations. The differentiation and functional properties of bone endothelial cells require cell-matrix signalling interactions. Loss of endothelial integrin β1 leads to endothelial cell differentiation defects and impaired postnatal bone growth, which is, in part, phenocopied by endothelial cell-specific laminin α5 mutants. Our work outlines fundamental principles of vessel formation and endothelial cell differentiation in the developing skeletal system.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adipokines / metabolism
  • Animals
  • Apelin
  • Bone and Bones / blood supply
  • Bone and Bones / cytology*
  • Bone and Bones / diagnostic imaging
  • Capillaries / cytology
  • Cell Adhesion
  • Endothelial Cells / metabolism*
  • Extracellular Matrix / metabolism*
  • Flow Cytometry
  • Immunohistochemistry
  • Integrases / metabolism
  • Integrin beta1 / metabolism
  • Intercellular Signaling Peptides and Proteins / metabolism
  • Mice, Inbred C57BL
  • Mice, Mutant Strains
  • Neovascularization, Physiologic
  • Osteogenesis*
  • Phenotype
  • Signal Transduction*
  • X-Ray Microtomography


  • Adipokines
  • Apelin
  • Apln protein, mouse
  • Integrin beta1
  • Intercellular Signaling Peptides and Proteins
  • Cre recombinase
  • Integrases