Mangiferin antagonizes TNF-α-mediated inflammatory reaction and protects against dermatitis in a mice model

Int Immunopharmacol. 2017 Apr;45:174-179. doi: 10.1016/j.intimp.2017.02.014.

Abstract

This study aimed to investigate whether mangiferin played a protective role in a well-established dermatitis mouse model and tumor necrosis factor alpha (TNF-α)-induced RAW264.7 macrophages. Contact dermatitis is an inflammatory skin disease in the clinic, while its underlying mechanism still remains to be elucidated. Mangiferin, 1,3,6,7-tetrahydroxyxanthone-C2-β-d-glucoside (C-glucosyl xanthone), a natural antioxidant that was reported to inhibit inflammatory reactions, has been recently proved to be a potential therapy for inflammation. As a result, the oxazolone-induced dermatitis mice models were established to explore whether mangiferin has an anti-inflammatory role in vivo. The phosphate-buffered saline treatment groups showed emblematic skin inflammation, whereas the administration of mangiferin obviously inhibited dermatitis in the mice models. Furthermore, exogenous mangiferin alleviated the inflammatory reaction in TNF-α-induced macrophages by suppressing the production of inflammation- and oxidative stress-associated molecules. Also, mangiferin treatment repressed the activation of nuclear factor-kappaB signaling pathway. To sum up, mangiferin could provide a new target for the therapy and prevention of skin inflammation.

Keywords: Dermatitis; Inflammation; Mangiferin; NF-κB signaling pathway; RAW264.7 macrophage; TNF-α.

MeSH terms

  • Animals
  • Anti-Inflammatory Agents / therapeutic use*
  • Dermatitis, Contact / drug therapy*
  • Disease Models, Animal
  • Humans
  • Inflammation / drug therapy*
  • Macrophages / drug effects*
  • Macrophages / immunology
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Oxazolone
  • RAW 264.7 Cells
  • Signal Transduction / drug effects
  • Skin / drug effects*
  • Skin / immunology
  • Tumor Necrosis Factor-alpha / immunology
  • Xanthones / therapeutic use*

Substances

  • Anti-Inflammatory Agents
  • Tumor Necrosis Factor-alpha
  • Xanthones
  • Oxazolone
  • mangiferin