Expression of binding sites for Dolichos biflorus agglutinin at the apical aspect of collecting duct cells in rat kidney

Cell Tissue Res. 1987 Sep;249(3):481-5. doi: 10.1007/BF00217319.

Abstract

To identify precisely the structural and functional cell type in the collecting duct of the rat kidney expressing binding sites for Dolichos biflorus agglutinin (DBA), we stained serial paraffin sections of kidney with horseradish peroxidase-labeled DBA and with immunocytochemical methods for localizing (Na+ + K+)-ATPase and carbonic anhydrase II (CA II), enzymes found preferentially in principal and intercalated cells, respectively. Most principal cells expressing a strong basolateral staining for (Na+ + K+)-ATPase showed binding sites for DBA at their luminal surfaces. However, a minority of cells rich in CA II and showing morphologic characteristics of intercalated cells also expressed DBA binding sites at their luminal surface and apical cytoplasm. These data suggest that DBA cytochemistry can provide a useful tool for studying the functional polarity of the main cell types of the collecting duct of the rat kidney.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Immune Sera
  • Immunohistochemistry
  • Kidney Cortex / enzymology
  • Kidney Cortex / immunology*
  • Kidney Medulla / enzymology
  • Kidney Medulla / immunology*
  • Kidney Tubules / enzymology
  • Kidney Tubules / immunology*
  • Male
  • Mitogens / metabolism*
  • Rats
  • Rats, Inbred Strains
  • Receptors, Mitogen / metabolism*
  • Sodium-Potassium-Exchanging ATPase / metabolism

Substances

  • Dolichos biflorus lectin receptor
  • Immune Sera
  • Mitogens
  • Receptors, Mitogen
  • Sodium-Potassium-Exchanging ATPase