Abstract
We investigated the effect of disulfiram (Antabuse) on the activity of alcohol dehydrogenase (EC 1.1.1.1) in vitro. We observed a time-dependent inhibition of this dehydrogenase by disulfiram and diethyldithiocarbamate similar to that obtained for aldehyde dehydrogenase (EC 1.2.1.3). These results suggest a possible explanation for various side effects observed in the clinical use of Antabuse.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Alcohol Dehydrogenase / antagonists & inhibitors*
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Aldehyde Dehydrogenase / antagonists & inhibitors
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Animals
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Disulfiram / pharmacology*
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Dithiothreitol / pharmacology
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Ditiocarb / pharmacology
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Horses
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Kinetics
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Liver / enzymology
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Serum Albumin / metabolism
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Time Factors
Substances
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Serum Albumin
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Ditiocarb
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Alcohol Dehydrogenase
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Aldehyde Dehydrogenase
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Dithiothreitol
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Disulfiram