Induced circularly polarized luminescence for revealing DNA binding with fluorescent dyes

J Pharm Biomed Anal. 2017 Sep 10:144:6-11. doi: 10.1016/j.jpba.2017.02.010. Epub 2017 Feb 12.

Abstract

To the best of our knowledge this is the first report on the application of induced circularly polarized luminescence (CPL) for sensing the binding of fluorescent dyes to double stranded DNA. Using Thiazole Orange (TO) and 4',6-diamidino-2-phenylindole (DAPI) as models, we show utility and limitations of CPL in DNA binding studies. The results obtained indicate that CPL can be used as a new chiroptical tool for this purpose, however, special attention while recording CPL data must be used, in order to exclude measurement artefacts caused by linear polarization components.

MeSH terms

  • DNA / chemistry*
  • Fluorescent Dyes
  • Luminescence

Substances

  • Fluorescent Dyes
  • DNA