Olegusella massiliensis gen. nov., sp. nov., strain KHD7T, a new bacterial genus isolated from the female genital tract of a patient with bacterial vaginosis

Anaerobe. 2017 Apr:44:87-95. doi: 10.1016/j.anaerobe.2017.02.012. Epub 2017 Feb 20.

Abstract

Strain KHD7T, a Gram-stain-positive rod-shaped, non-sporulating, strictly anaerobic bacterium, was isolated from the vaginal swab of a woman with bacterial vaginosis. We studied its phenotypic characteristics and sequenced its complete genome. The major fatty acids were C16:0 (44%), C18:2n6 (22%), and C18:1n9 (14%). The 1,806,744 bp long genome exhibited 49.24% G+C content; 1549 protein-coding and 51 RNA genes. Strain KHD7T exhibited a 93.5% 16S rRNA similarity with Olsenella uli, the phylogenetically closest species in the family Coriobacteriaceae. Therefore, strain KHD7T is sufficiently distinct to represent a new genus, for which we propose the name Olegusella massiliensis gen. nov., sp. nov. The type strain is KHD7T.

Keywords: Bacterial vaginosis; Culturomics; Genome; Olegusella massiliensis; Taxono-genomics; Vaginal flora.

Publication types

  • Case Reports

MeSH terms

  • Actinobacteria / classification*
  • Actinobacteria / genetics
  • Actinobacteria / isolation & purification*
  • Actinobacteria / physiology
  • Adult
  • Anaerobiosis
  • Bacterial Typing Techniques
  • Base Composition
  • Cluster Analysis
  • Cytosol / chemistry
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • DNA, Ribosomal / chemistry
  • DNA, Ribosomal / genetics
  • Fatty Acids / analysis
  • Female
  • Genome, Bacterial
  • Humans
  • Microscopy
  • Phylogeny
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA
  • Vagina / microbiology*
  • Vaginosis, Bacterial / microbiology*

Substances

  • DNA, Bacterial
  • DNA, Ribosomal
  • Fatty Acids
  • RNA, Ribosomal, 16S