Cloning and expression of the gene for a fibronectin-binding protein from Staphylococcus aureus

EMBO J. 1987 Aug;6(8):2351-7.


The gene encoding the fibronectin-binding protein (FNBP) from Staphylococcus aureus strain 8325-4 was isolated from a gene bank in pBR322. The original clone, containing a 6.5-kb insert, gave a functional product present in the periplasm of Escherichia coli. Analysis of polypeptides isolated after affinity chromatography on fibronectin-Sepharose followed by ion-exchange chromatography revealed two gene products, 87 and 165 kd in mol. wt. The amino acid compositions of these two polypeptides and a native FNBP from S. aureus strain Newman were very similar. Antibodies raised against the native FNBP from strain Newman precipitated the 125I-labelled 165-kd polypeptide, and unlabeled 165- and 87-kd polypeptides as well as native FNBP inhibited the immunoprecipitation reactions. The region of the fnbp-gene encoding the fibronectin-binding activity has been identified and subcloned in an expression vector based on the staphylococcal protein A gene. The resulting product in E. coli is an extracellular fusion protein consisting of two IgG-binding domains of protein A followed by a fibronectin-binding region. The fusion protein binds to fibronectin and completely inhibits the binding of fibronectin to intact cells of S. aureus.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Cloning, Molecular*
  • DNA Restriction Enzymes
  • Escherichia coli / genetics
  • Fibronectins / metabolism
  • Genes*
  • Genes, Bacterial*
  • Molecular Weight
  • Plasmids
  • Receptors, Fibronectin
  • Receptors, Immunologic / genetics*
  • Receptors, Immunologic / metabolism
  • Staphylococcus aureus / genetics*
  • Transcription, Genetic*


  • Fibronectins
  • Receptors, Fibronectin
  • Receptors, Immunologic
  • DNA Restriction Enzymes