Development of drug delivery systems for taxanes using ionic gelation of carboxyacyl derivatives of chitosan

Yury A Skorik; Anton A Golyshev; Andreii S Kritchenkov; Ekaterina R Gasilova; Daria N Poshina; Amal J Sivaram; Rangasamy Jayakumar

doi:10.1016/j.carbpol.2017.01.025

Development of drug delivery systems for taxanes using ionic gelation of carboxyacyl derivatives of chitosan

Carbohydr Polym. 2017 Apr 15:162:49-55. doi: 10.1016/j.carbpol.2017.01.025. Epub 2017 Jan 6.

Authors

Yury A Skorik¹, Anton A Golyshev², Andreii S Kritchenkov³, Ekaterina R Gasilova², Daria N Poshina², Amal J Sivaram⁴, Rangasamy Jayakumar⁴

Affiliations

¹ Institute of Macromolecular Compounds of the Russian Academy of Sciences, Bolshoi pr. VO 31, St. Petersburg 199004, Russian Federation; Institute of Chemistry, St. Petersburg State University, Universitetskii pr. 26, Petrodvorets, St. Petersburg 198504, Russian Federation. Electronic address: yury_skorik@mail.ru.
² Institute of Macromolecular Compounds of the Russian Academy of Sciences, Bolshoi pr. VO 31, St. Petersburg 199004, Russian Federation.
³ Institute of Macromolecular Compounds of the Russian Academy of Sciences, Bolshoi pr. VO 31, St. Petersburg 199004, Russian Federation; Institute of Chemistry, St. Petersburg State University, Universitetskii pr. 26, Petrodvorets, St. Petersburg 198504, Russian Federation.
⁴ Amrita Centre for Nanosciences & Molecular Medicine, Amrita Institute of Medical Sciences and Research Centre, Amrita Viswa Vidyapeetam University, Kochi 682041, India.

PMID: 28224894
DOI: 10.1016/j.carbpol.2017.01.025

Abstract

Nanoparticles of two chitosan derivatives - N-succinyl-chitosan (SC) and N-glutaryl-chitosan (GC) - were developed as passive transport systems for taxanes (paclitaxel and docetaxel) using an ionic gelation technique with sodium tripolyphosphate. These nanoparticles had an apparent hydrodynamic diameter of 300-350nm, a ζ-potential of 25-31mV, an encapsulation efficiency of 21-26%, and a drug loading efficiency of 6-13%. DLS and SLS analysis shows that the nanoparticles have a unimodal size distribution and spherical form. Drug release kinetics of the taxane-loaded nanoparticles demonstrates that more than 50% of the loaded taxane could be released upon the degradation of the nanoparticles after targeted delivery. The drug-loaded SC and GC nanoparticles exhibit high cytotoxicity towards AGS cancer cell lines and their antitumor activity is consequently enhanced when compared with free taxanes.

Keywords: Docetaxel; Drug delivery; Glutaryl-chitosan; Ionic gelation; Paclitaxel; Succinyl-chitosan.

MeSH terms

Chitosan / chemistry*
Drug Carriers / chemistry
Drug Delivery Systems*
Nanoparticles / chemistry
Particle Size
Taxoids / administration & dosage*

Substances

Drug Carriers
Taxoids
Chitosan