In situ protein-DNA interactions at a dioxin-responsive enhancer associated with the cytochrome P1-450 gene

Mol Cell Biol. 1987 Aug;7(8):3008-11. doi: 10.1128/mcb.7.8.3008-3011.1987.

Abstract

We used an in situ exonuclease III protection technique (C. Wu, Nature [London] 309:229, 1984) to analyze protein-DNA interactions at a dioxin-responsive enhancer. Our results imply that the 2,3,7,8-tetrachlorodibenzo-p-dioxin-receptor complex interacts with the dioxin-responsive enhancer to activate transcription of the cytochrome P1-450 gene.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Base Sequence
  • Chromatin / drug effects
  • Chromatin / ultrastructure
  • Cytochrome P-450 Enzyme System / genetics*
  • Deoxyribonucleoproteins / metabolism*
  • Dioxins / pharmacology*
  • Enhancer Elements, Genetic / drug effects*
  • Exodeoxyribonucleases
  • Genes / drug effects*
  • Genes, Regulator / drug effects
  • Liver Neoplasms, Experimental / metabolism
  • Mice
  • Polychlorinated Dibenzodioxins / pharmacology*
  • Receptors, Aryl Hydrocarbon
  • Receptors, Drug / metabolism
  • Transcription, Genetic

Substances

  • Chromatin
  • Deoxyribonucleoproteins
  • Dioxins
  • Polychlorinated Dibenzodioxins
  • Receptors, Aryl Hydrocarbon
  • Receptors, Drug
  • Cytochrome P-450 Enzyme System
  • Exodeoxyribonucleases
  • exodeoxyribonuclease III